The move comes amid continuing protests by pro-democracy campaigners in Hong Kong who have been demanding the right to choose their own leaders without interference from Beijing.
The Foreign Affairs Committee, a panel of lawmakers who scrutinise the Foreign Office's work, is looking into Britain's relations with the Chinese special administrative region 30 years after the 1984 Joint Declaration, which set out the terms of the 1997 handover of Hong Kong.
Under the Joint Declaration Hong Kong is governed according to a "one country, two systems" principle, enjoying freedoms not known on the mainland.
But when asked about the refusal Beijing's foreign ministry spokeswoman Hua Chunying said the situation in the city falls within China's domestic affairs and insisted foreign countries had no right to "interfere".
The delegation's attempt to travel was "overtly confrontational and it is not conducive to the development of China-UK relations", Hua told a regular press briefing.
British citizens do not require visas to visit Hong Kong, but Hua said: "It is the sovereign right of every country to decide whether or not to issue a visa or what kind of visa to issue.
"China has repeatedly expressed to Britain that we are opposed to the so-called investigation by the group sent by the British lower house of parliament."
Another group of British lawmakers last week cancelled a visit to mainland China after an MP who spoke up for Hong Kong's autonomy was not granted a visa.
It also comes after an announcement Thursday that Prince William will visit China in March on behalf of the British government.
