The declaration by Wang comes after a sweeping anti-corruption drive under Xi has targeted a swathe of prominent cadres and low-level officials.
The campaign has seen thousands dismissed from their posts, with some receiving lengthy prison terms, but critics say it has been used to purge Xi's political rivals.
An investment company owned by Xi's brother-in-law, Deng Jiagui, bought shares in Wanda's commercial property subsidiary in a private placement in 2009, Wang told a Harvard Business School forum on Thursday.
"He sacrificed the opportunity to realise a huge return in investment," he said according to a press release Friday.
"This incident demonstrates that President Xi is not only strict in managing our country but is even more scrupulous when it comes to family affairs."
The comments were made in response to a question about a New York Times article alleging that friends and relatives of China's top leadership made more than $1 billion from investments in Wanda's commercial property and cinema companies.
"There are all kinds of such rumours flowing around," spokesman Lu Kang told a regular briefing. "I don't think they need a comment."
Wang has sought to play down overseas speculation on the link between his success and government ties, which in China can be crucial to seal land deals with local authorities.
"Wanda has no political affiliation," he said at the forum.
Family members and friends of Xi, his predecessor Hu Jintao, former premier Wen Jiabao and other top officials all had links to companies controlled by Wang, according to the New York Times.
The son of a Red Army captain, Wang was a soldier before he founded Wanda in the 1980s, building it up with military discipline.
Its website says it is Asia's largest private property owner, and the Wanda Commercial flotation raised USD 3.7 billion, valuing that unit alone at USD 24 billion.
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