About a fifth of male river fish are displaying feminised traits and even producing eggs, researchers said.
Some have reduced sperm quality and display less aggressive and competitive behaviour, making them unlikely to breed successfully.
The chemicals causing these effects include ingredients in the contraceptive pill, by-products of cleaning agents, plastics and cosmetics.
"We are showing that some of these chemicals can have much wider health effects on fish that we expected," said Charles Tyler, of the University of Exeter in the UK.
According to the study, 20 per cent of male freshwater fish at 50 sites had feminine characteristics.
More than 200 chemicals from sewage plants have been identified with oestrogen-like effects and drugs such as antidepressants are also altering fish's natural behaviour, researchers said.
"Other research has shown that many other chemicals that are discharged through sewage treatment works can affect fish, including antidepressant drugs that reduce the natural shyness of some fish species, including the way they react to predators," Tyler said.
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