In its first reaction a day after scores were hurt in clashes in Catalonia, the European Commission said the referendum was "not legal" under Spanish law and was an "internal matter" for Spain.
An independent Catalonia would also have to leave the EU if it did vote for independence in a legal referendum, European Commission spokesman Margaritis Schinas said.
"Beyond the purely legal aspects of this matter, the commission believes that these are times for unity and stability, not divisiveness and fragmentation," Schinas said in a statement to reporters.
"We trust the leadership of Prime Minister Mariano Rajoy to manage this difficult process in full respect of the Spanish constitution and of the fundamental rights of citizens enshrined therein."
Spain said today it will do "everything within the law" to prevent Catalonia from declaring independence, a day after Catalonia's regional government declared victory in the referendum.
"Under the Spanish Constitution, yesterday's vote in Catalonia was not legal," Schinas added.
"For the European Commission, as President (Jean-Claude) Juncker has reiterated repeatedly, this is an internal matter for Spain that has to be dealt with in line with the constitutional order of Spain.
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