Four of the victims, three women and one man, died at the scene while another woman died in hospital, Allegheny County police said in a statement.
Two men are in critical condition and a woman is in stable condition following the incident at a home, they said.
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No one has been taken into custody.
Police said shots were fired from an alley and that when partygoers tried to seek cover in the home, someone on the side or backyard adjacent to the residence fired at the back porch.
All four victims who perished at the scene died on the back porch.
Neighbourhood resident Kayla Alexander told local broadcaster WPXI that the street where the shooting occurred is normally quiet and filled with playing children.
The mass shooting is the latest to rock the United States, where such attacks have become commonplace.
Gun violence is responsible for some 30,000 deaths per year in the country, with 330 mass shooting incidents recorded last year.
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