The Comoros, which has referred the case to the ICC, "asks the Chamber to request the Prosecutor to reconsider her decision not to open an investigation," its lawyers said in papers filed before the Hague-based court.
Ten Turkish activists died after Israeli commandos staged a botched pre-dawn raid on the six-ship flotilla seeking to break Israel's blockade of the Gaza Strip in May 2010.
The ship on which the activists sailed, the MV Mavi Marmara is registered in the tiny Indian Ocean island country, which has been a state party to the ICC since 2006.
Bensouda said any potential cases arising from an investigation into the incident would not be of "sufficient gravity" to justify further ICC action.
But the Comoros' lawyers said "those on the flotilla are all entitled to the ICC's condemnation of impunity and to its sanctioning of individuals who might have hoped to enjoy impunity."
Bensouda failed to "take relevant matters" into consideration, including the broader context of the Israeli-Palestinian conflict, the lawyers said.
Israel imposed the blockade on Gaza in 2006 and strengthened it a year later when the Islamist Hamas movement took control of Gaza, then eased it somewhat following the international outcry over the killing of the Turkish activists.
The ICC, which was set up in 2002, tries persons accused of the world's worst crimes, namely genocide, crimes against humanity and war crimes.
Bensouda earlier this month launched a preliminary probe into possible war crimes committed against Palestinians including during last year's Gaza offensive.
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