Gayathiri Bose, a manager at a transport company who has a three-year-old child and a seven-month-old baby, said she has filed a complaint with German police alleging she was told to squeeze her breast at airport security to prove she was lactating.
She told the BBC she was "humiliated" and "very traumatised" by the experience and would explore formal legal action.
She said police at Frankfurt Airport were suspicious because she was carrying a breast pump but travelling without her baby.
But they said such measures were "clearly" not part of routine procedure.
Bose, who was travelling alone, said she was on her way to board a flight to Paris last Thursday when she was stopped at the security screening station.
She said that after her carry-on bag, which contained her breast pump, went through the X-ray machine, she was taken aside for questioning.
"[They had] an incredulous tone. 'You are breastfeeding? Then where is your baby? Your baby is in Singapore?'," she said about the 45-minutue episode.
They kept her passport and she was then led to a room by a female police officer for further questioning, she said.
Bose said she complied and squeezed her breast. "I was just in shock, I was going through the motions. I was all by myself as well, and wasn't sure what would happen to me if they decided to make trouble for me."
"It was only when I came out of the room that I began to slowly understand what had just happened. I just started to cry, I was terribly upset."
She said officials then tested and cleared the pump before returning her passport, and she was allowed to board her plane to Paris.
"The officer just said, 'Okay it is over now, please go'.
Christian Altenhofen, spokesman for the German federal police unit at Frankfurt Airport, told the BBC that he could not comment on the incident "for reasons of data protection".
He added: "If a suspected explosive is detected at an air safety control point, the baggage and the person must be searched. The measures you have described for a breastfeeding mother are clearly not included.
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