The villagers were reportedly annoyed over the addition of one more toll booth at Waghote village on the Wada Manor road under Wada taluka of Thane, taking the total number of booths to 18 in the district.
They were also angry over the toll collection allegedly being started even as the work on the road there was yet to be completed, police said.
The agitation was carried out today by local Congress workers, led by Nilesh Sambre, they said.
While the villagers claimed that two persons were injured in the firing, police were yet to confirm it.
They said the situation was under control and the traffic on the highway had resumed.
The toll collection centre had started functioning earlier this month.
Police said offences were being registered in connection with the incident.
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