King Hezekiah deliberately defiled the eighth centry BC shrine at the door to the ancient city of Lachish, as part of a campaign to centralise Jewish ritual in Jerusalem, the Israel Antiquities Authority said.
"A toilet was installed in the holy of holies as the ultimate desecration of that place," the IAA said in a statement.
"A stone fashioned in the shape of a chair with a hole in its centre was found in the corner of the room."
"And they demolished the pillar of Baal, and demolished the house of Baal, and made it a latrine to this day," the statement quoted the Bible as saying.
But laboratory tests suggest the stone toilet at the Lachish gate was never used, the IAA said.
This showed its placement was "symbolic, after which the holy of holies was sealed until the site was destroyed".
The city gate was first located "decades ago", the IAA said, but was only fully exposed in early 2016.
"The excavation revealed destruction layers in the wake of the defeat, including arrowheads and sling stones, indicative of the hand-to-hand combat that occurred in the city's gatehouse," it said.
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