The rebels were killed in fighting, car bombings and also summary executions by forces loyal to the Islamic State of Iraq and the Levant.
ISIL, an Al-Qaeda affiliate, moved into Syria in late spring last year amid the armed uprising against President Bashar al-Assad.
The latest deaths come a third day into fighting that began when rebels, including Islamists, attacked checkpoints and bases manned by ISIL, which is accused of horrific abuses in areas where its fighters operate.
At least nine ISIL fighters were also killed today, the Britain-based Observatory said.
Most of the non-ISIL fatalities were in the northern provinces of Aleppo and Idlib, where the fighting has been focused since Friday.
Among the dead were "seven who were summarily executed by ISIL" in Harem in Idlib.
Clashes also erupted in Raqa and Hama provinces today, in a sign the fighting was spreading beyond the northern provinces.
Scores of fighters on both sides have been killed in the past three days, according to the Observatory.
The Observatory also said ISIL fighters were laying down their arms and withdrawing from some areas including Daret Izza, without giving further details.
In Atareb, also in Aleppo province, rebels raised the green, white, black and red flag of the opposition, and brought down the flag flown by ISIL.
The Islamic Front, the Syrian Revolutionaries Front and the nascent Army of Mujahideen -- rebel alliances -- are all battling ISIL.
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