Wendy Baker, now 75, has put up the priced possession for auction, reported Daily Express.
Baker received the gift from the late Beatle star during a performance by the band at Soho club in London in 1966.
Bournemouth-born Baker said, "It is hard to imagine someone as cool as John doing the conga, but it happened."
Darren Julien, who will sell the specs in Los Angeles on November 7, said: "It is rare to find a pair of Lennon's worn glasses.
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