Zeng Feiyang, director of labour rights group the Panyu Workers' Centre, and activist Zhu Xiaomei were both placed under criminal detention Friday, Hong Kong-based non-governmental organisation China Labour Bulletin (CLB) reported.
CLB said Zhu Xiaomei has a one-year-old baby girl who has never been separated from her mother.
Another local activist, He Xiaobo, was detained earlier on Friday, CLB said. Chinese NGO Weiquanwang (Rights Defence Network) reported that He worked with an organisation which helps workers suffering from occupational injuries.
Zeng has been helping workers in the southern province of Guangdong win payment of wages and unpaid benefits in disputes against employers.
Both Zeng and Zhu are accused of illegally "gathering a crowd to disturb social order", according to Weiquanwang. Such charges are often invoked by the authorities against those it considers troublemakers.
Independent trade unions are banned in China, with only the official All-China Federation of Trade Unions legally recognised. However, critics say it often fails to assist workers in disputes.
"This campaign of arrests against groups of workers' rights activists in Guangdong is unprecedented," CLB spokesman Geoffrey Crothall told AFP, adding it was "very alarming".
The crackdown comes as Guangdong, one of China's richest provinces, confronts growing economic challenges as many factories in the manufacturing hub of the Pearl River Delta close or relocate to cheaper Chinese provinces -- or countries such as Vietnam where labour costs are lower.
Workers are often left with unpaid wages and no redundancy pay.
The detention of the three activists was part of a wider operation and the fate of four others -- Peng Jiayong, Meng Han, Deng Xiaoming and Bei Guo -- remains unknown, CLB reported.
Since President Xi Jinping came to power in 2013, Beijing has taken a harder line towards rights groups. Xi has overseen a crackdown on dissent that has seen hundreds of lawyers, activists and academics detained in recent years, with dozens jailed.
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