The 'Living for Love' singer will be interviewed by Ross for the first time since 2003 in March, reported Digital Spy.
The special edition of the chat show will focus exclusively on Madonna, as she sits down to discuss her new album Rebel Heart.
She will also perform two songs from her forthcoming record on the show.
"Madonna is remarkable. A bold, daring, uncompromising superstar who shot to the top back in the '80s and has remained there ever since," Ross said.
"I am hoping this interview will be as memorable and revealing as the first time I sat down with her over 20 years ago," Ross added.
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