The Harley Davidson FLH Electra-Glide motorcycle was purchased by Brando in 1970 and it is probably the only "Brando motorcycle available. The auction takes place on June 27 in Beverly Hills.
The bike is estimated to be worth between USD 200,000 to 400,000, reported People magazine.
"My father was bigger than life and everything he did was often celebrated by fans around the globe," Miko Brando, the actor's son, said.
"My hope is that many of the items coming up for auction this weekend at Julien's Auctions will offer a small glimpse into the life of my father, who happened to also be one of the world's most loved and famous actors of our time."
The actor, however, rode a 1950 6T Triumph Thunderbird bike in the movie.
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