The tanks crossed into the village from Elbeyli in the Turkish province of Kilis to provide military support to Syrian opposition fighters after ridding northern villages of extremists in its "Euphrates Shield" operation launched on August 24, state-run Anadolu news agency said.
At least 20 tanks, five armoured personnel carriers, trucks and other armoured vehicles crossed the border after noon, Dogan news agency said.
Turkish Firtina howitzers fired on IS targets as the fresh armoured contingent advanced, Dogan said.
This is Ankara's most ambitious operation during the five-and-a-half-year Syria conflict, backed by the tanks as well as war planes and special forces providing support to rebels.
The goal is to remove IS from its border and to halt the westward advance of the Kurdish People's Protection Militia (YPG).
Ahmed Othman, a commander in pro-Turkey rebel group Sultan Murad, told AFP in Beirut that his group was now "working on two fronts in al-Rai, south and east, in order to advance towards the villages recently liberated from IS west of Jarabulus".
After the Kurds' success in Manbij, they said they wanted to advance and link their other two 'cantons' in northern Syria, Kobane and Afrin.
But President Recep Tayyip Erdogan said yesterday that Turkey would not allow the group to create a "terror corridor".
Ankara sees the YPG as a terror organisation linked to Kurdish separatist rebels in southeast Turkey but the United States has provided training and equipment to the group.
Within 14 hours on August 24, Turkish-backed Syrian rebels recaptured the border town of Jarabulus from IS and continued to make gains in villages nearby.
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