The US State Department said it was aware of the reports, but there was no official confirmation of the arrest, which would come at a tense time in relations between Pyongyang and Washington.
Yonhap quoted sources as saying the man, identified only by his surname Kim, was arrested on Friday at Pyongyang International Airport on his way out of the country.
It said Kim, aged in his late 50s and a former professor at China's Yanbian University of Science and Technology, had been involved in aid programmes for the North.
"We are aware of reports that a US citizen was detained in North Korea," a State Department official said in Washington.
Due to the lack of diplomatic relations, "In cases where US citizens are reported to be detained in North Korea, we work with the Swedish embassy, which serves as the United States' Protecting Power in North Korea. Due to privacy considerations, we have no further comment," the official said.
South Korea's National Intelligence Service as well as the unification and foreign ministries said they could not confirm the report.
"The reason North Korea is not saying anything yet is because it is not done with the investigations," Ahn Chan-il, a former defector, told AFP.
"It is important for them to hold a US citizen hostage at this point to prevent Washington from carrying out a decapitation of Kim Jong-Un," Ahn said, referring to the North's fears that the US plans a secret military strike to topple its leader.
"It's also a resolve to point a double-action revolver against the US and China because he is a US citizen who worked in China."
Vice President Mike Pence, during a regional tour last week, warned that "all options are on the table" to curb the North's nuclear ambitions as fears grow it may be planning another atomic test.
Two other US citizens -- college student Otto Warmbier and Korean-American pastor Kim Dong-Chul -- are currently being held in the North after being sentenced to long prison terms.
The pastor Kim was sentenced last year to 10 years of hard labour for spying.
North Korea has arrested and jailed several US citizens in the past decade, often releasing them only after high- profile visits by current or former US officials.
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