The 35-year-old actress was recently spotted at Chiltern Firehouse in London last month "sans wedding ring", reported Ace Showbiz.
"She was trying to have a good time, but it seemed like her mind was somewhere else. She kept rubbing her empty ring finger. She looked really sad."
On what has drifted that pair apart, a source dished, "Their lives have been completely uprooted, and their relationship has taken a hit."
Portman and Millepied have been living in London during the production of her upcoming movie "Annihilation".
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