Until now, only a handful of people had laid eyes on the original 1895 document that has been stashed away in a safe at the Nobel Foundation in Stockholm.
The Nobel Museum has changed all that, putting it on public display as part of its new exhibition "Legacy".
"The exhibition draws attention to how important it is to pass things on. The will is the centrepiece. It is a simple document, but it still today provides the basis of our work with the (Nobel) prizes," exhibition curator Karin Jonsson tells AFP.
The Nobel prizes are "one of Sweden's biggest brands," notes a spokesman for the Swedish Institute, Sergio Guimaraes.
The will itself is written on four yellowed pages, with curlicued old-fashioned handwriting in black ink. It's smudged in places, and there are notes and additions running up and down the sides, tops and bottoms of the pages.
Nobel, the inventor of dynamite, details the creation of the Nobel prizes on 26 lines, or almost three-quarters of a page.
He stipulates that part of his fortune -- some 31.5 million kronor at the time, or the equivalent of more than 200 million euros (USD 220 million) today -- was to be placed in a fund, the interest on which was to be used to honour "those who, during the preceding year, shall have conferred the greatest benefit on mankind" in the fields of medicine, physics, chemistry, literature and peace.
The prize for economics was created in 1968 by Sweden's central bank, the Riksbank, to mark its tricentenary, and is also funded by the Riksbank.
Those 26 lines would change Nobel's reputation forever, and shine the spotlight on Sweden for years to come.
Gone is Nobel's image as a dynamite-producing friend of war -- now he is seen as a pacific philanthropist around the world.
"With these prizes, he wanted to show who he really was," explains Jean-Francois Battail, a professor emeritus in Scandinavian languages and literature at Paris' Sorbonne University.
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