Previous evidence from prehistoric rock art is inferred to show honey hunters and Pharaonic Egyptian murals show early scenes of beekeeping.
However, the close association between early farmers and the honeybee remained uncertain, researchers said.
Researchers at the University of Bristol in UK gathered together evidence for the presence of beeswax in the pottery vessels of the first farmers of Europe by investigating chemical components trapped in the clay fabric of more than 6,000 potsherds from over 150 Old World archaeological sites.
For example, beeswax was detected in cooking pots from an archaeological site in Turkey, dating to the seventh millennium BC - the oldest evidence yet for the use of bee products by Neolithic farmers.
"The most obvious reason for exploiting the honeybee would be for honey, as this would have been a rare sweetener for prehistoric people," said Melanie Roffet-Salque, Postdoctoral Research Assistant and lead author of the research paper.
"However, beeswax could have been used in its own right for various technological, ritual, cosmetic and medicinal purposes, for example, to waterproof porous ceramic vessels," said Roffet-Salque.
"The lack of a fossil record of the honeybee means it's ecologically invisible for most of the past 10,000 years," said Professor Richard Evershed from Bristol's Organic Geochemistry Unit.
"Although evidence from ancient Egyptian murals and prehistoric rock art suggests humankind's association with the honeybee dates back over thousands of years, when and where this association emerged has been unknown - until now," said Evershed.
"Our study is the first to provide unequivocal evidence, based solely on a chemical 'fingerprint', for the palaeoecological distribution of an economically and culturally important animal," he said.
The study was published in the journal Nature.
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