Star batsman Pietersen will be given time to prove his fitness for the match, which starts at the Manchester venue on Thursday, with Nottinghamshire's James Taylor added to the squad as cover.
England have also altered their bowling options by bringing in Monty Panesar as a second spinner and recalling fast bowler Chris Tremlett in place of seamers Steven Finn and Graham Onions.
Ashes-holders England, who lead the five-match series 2-0 after victories by 14 runs and 347-runs in the first and second Tests at Trent Bridge and Lord's respectively, will retain the urn if they win again at Old Trafford.
Alastair Cook (capt), Joe Root, Jonathan Trott, Kevin Pietersen, Ian Bell, Jonny Bairstow, Matt Prior (wkt), Tim Bresnan, Stuart Broad, Graeme Swann, James Anderson, James Taylor, Chris Tremlett, Monty Panesar.
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