Italian astronaut Paolo Nespoli, 60, admitted that despite the bird's eye view of Earth he too remained "perplexed", while American Mark Vande Hei said seeing the planet from space made them "realise how fragile we are".
The Argentine pontiff sat at a small desk inside the tiny city state, facing a wide-screen television on which the astronauts from America, Russia and Italy could be seen floating together in their blue suits.
"Good afternoon... or good evening. I imagine time passes differently at the space station, right?" the pope quipped.
A 30-second delay in the satellite feed gave Nespoli, Vande Hei, Americans Randolph Bresnik and Joseph Acaba, and Russians Sergej Nikolaevic Rjazanskij and Alexandr Misirkin time to weigh up life's biggest questions.
"Our aim here is to spread knowledge, (but) the more we learn, the more we realise we do not know," Nespoli admitted.
"I would like people like you, theologians, philosophers, poets, writers, to come to space to explore what it means to be a human in space," he said, as Francis nodded and smiled.
The crew took it in turns to get their philosophical caps on, and not even a technical hitch which temporarily interrupted the interview, or the lack of a Russian translator, could throw the space travellers off course.
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