Protest demonstrations were held in suburbs of Dindigul and in Thanjavur by locals, who blocked roads and demanded that the government immediately withdraw the hike.
In Chennai, DYFI activists staged protests.
The members of the public, comprising mainly daily wage earners, said the new fare implemented from yesterday took away a chunk of their meagre income.
Similar protests were held in other places like Chengam in Tiruvannamalai and Kulithalai in Karur district.
In several bus routes, passengers entered into arguments with conductors, who had a tough time convincing them about the new fare structure.
Near Ullundurpet in Villupuram district, passengers of a local bus refused to buy tickets as per the new fare structure and halted the bus, following which officials intervened to pacify them.
Yesterday too, members of the public had staged protests in some towns.
The bus fare hike has drawn flak from political parties, including the main opposition DMK, which has accused the government of being 'sadistic' and giving a 'thunderbolt' to the people.
The TMC said today said it would stage demonstrations across Tamil Nadu on January 29 against the hike, as well as for other issues.
Party President G K Vasan told reporters at Erode that he would lead the demonstration in the city that day.
All parties have demanded an immediate rollback of the hike.
Chief Minister K Palaniswamy said yesterday that the hike was effected with "anguish," which was inevitable due to a host of factors which includes increase in the cost of fuel, and maintenance.
"The government was pushed to increase the bus fare and there is no scope for a rollback," he had said.
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