"I hope that common sense will prevail, ... That we will work together normally and that we and our partners will not cause harm by poking at one another," Putin was quoted as saying by Russian news agencies during a visit to the eastern Siberian city of Yakutsk.
The remarks came a day after European Union leaders gave Russia a week to reverse course in Ukraine or face a new round of sanctions.
Russian officials have said previously that they were preparing countermeasures in case of new Western sanctions, after having last month banned most EU and US food imports.
But Foreign Minister Sergei Lavrov earlier today said that Russia would not "slam the door shut" on Western trade nor exit from the World Trade Organisation.
"We will, above all, proceed from our own interests, defend our economy, defend our social system, defend our businesses..." Lavrov told Moscow university students.
It also sank to a five-month low of 49.1795 to the euro.
Russian stocks were also lower in early afternoon trading, with the ruble-denominated MICEX index shedding 0.33 per cent and the dollar-denominated RTS slumping 1.30 per cent after sharper falls on Friday.
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