Ramkumar lost 3-6 6-2 6-7(4) to Argentine 30th seed Facundo Bagnis in the second round of the qualifying event of the last Grand Slam tournament of the 2015 season.
Somdev Devvarman, Yuki Bhambri and Saketh Myneni had exited after losing their respective first round matches.
India's challenge will be restricted to the doubles event with seasoned Leander Paes, Wimbledon champion Sania Mirza and Rohan Bopanna playing with their respective partners.
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