Sir Alex Younger, chief of the Secret Intelligence Service - known as MI6, made the revelation in an attempt to encourage more females to join the secret service.
Speaking at the Women in IT awards yesterday, Alex said: "The real-life Q is looking forward to meeting you and I'm pleased to report that the real-life Q is a woman."
Actors who have played the role of Q in the Bond series based on Ian Fleming's writings include Ben Whishaw, John Cleese and before that World War II veteran Desmond Llewelyn, who made the part his own for 17 films.
"It leads to a stereotype which is of a particular kind or a particular sort of person that will join MI6 - whether they're really posh or going to Oxford or whatever it is. I'm none of those things, by the way," he said.
The MI6 chief said he wanted to make the service even more diverse which would bring a full range of skills to the service and improve its decision-making.
He added: "The more different people you have in the room, in these high-pressure circumstances in which we operate, the better the decisions.
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