Ryan spoke a day after a special House panel issued a report criticizing the organization, which provides birth control, abortions and various women's health services, for its practices regarding providing tissue from aborted fetuses to researchers.
The Wisconsin lawmaker's comments, while expected, were the first official word that repeal legislation would also renew the congressional assault on the group.
"The Planned Parenthood legislation would be in our reconciliation bill," Ryan said.
Last year's Obamacare repeal measure also contained the effort to defund the group, which receives government reimbursements from the Medicaid program for non-abortion health services to low-income women.
The defunding measure would take away roughly USD 400 million in Medicaid money from the group in the year after enactment, according to the nonpartisan Congressional Budget Office, and would result in roughly 400,000 women losing access to care.
One factor is that being enrolled in Medicaid doesn't guarantee access to a doctor, so women denied Medicaid services from Planned Parenthood may not be able to find replacement care.
President-elect Donald Trump sent mixed signals during the campaign about the 100-year-old organization.
He said "millions of women are helped by Planned Parenthood," but he also endorsed efforts to defund the group. Trump once described himself as "very pro-choice," but now opposes abortion rights.
Planned Parenthood is loathed by anti-abortion activists who are the backbone of the GOP coalition. Polls, however, show that the group is favorably viewed by a sizable majority of Americans -- 59 per cent in a Gallup survey last year, including more than one-third of Republicans.
The defunding effort could also complicate Obamacare repeal in the Senate, where at least one GOP member -- Susan Collins of Maine -- cited the defunding language in opposing the repeal effort in late 2015.
Asked yesterday about party efforts to tie the effort to defund Planned Parenthood to Obamacare repeal, Collins said, "that's of concern to me as well, but I don't want to prejudge what's in the ... Bill.
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