Beijing has forced dozens of South Korean retail stores in the country to shut their doors and banned Chinese tour groups from visiting the South.
The moves are seen in the South as economic retaliation for the installation of the Terminal High Altitude Area Defence (THAAD) system to guard against threats from the nuclear-armed North, which Beijing sees it as a threat to its own military.
The trade ministry asked China to "comply with its obligations" and raised the issue of possible violation of WTO rules with the Geneva-based organisation, minister Joo Hyung-Hwan told Seoul lawmakers.
China is the South's largest trading partner and a major consumer of South Korean electronic gadgets, cosmetics and cultural products including popular TV dramas.
Lotte - the South's number five business group focused on retail and hotel chains - has been hit hardest after it agreed to provide land for the US missiles.
Nearly 90 per cent of its Chinese Lotte Mart stores have been forced to close either by authorities or angry demonstrations amid growing boycott threats in China against other South Korean firms.
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