Professor Gilles Edan, the chief neuroscientist at Rennes Hospital, spoke at a press conference Friday in the western French city.
French Health Minister Marisol Tourainealso told reporters that all those hospitalized were healthy men when the trial began on January 7.
She said one man who has now been classified as brain dead was admitted to the hospital on Sunday.
The French prosecutor's office has opened an investigation into what the Health Ministry is calling a "serious accident during a clinical test" at the Biotrial laboratory in Rennes.
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