Besides, profit-booking in with stocks of metals, PSU, banking, power, infrastructure, auto and healthcare sectors, negatively impacted trading sentiments.
The 30-share barometer, which had retreated from its record-high by falling 11.86 points in yesterday's highly volatile session, fell further by 152.74 points, or 0.51 per cent, to 29,406.44.
The National Stock Exchange index Nifty also slipped from record-high and shed 52.80 points, or 0.59 per cent, to 8,861.50.
The gauge had closed at a lifetime high of 8,914.30 after climbing to an intra-day record-high of 8,985.05 yesterday.
Besides, a weakening trend at other Asian markets following overnight losses in US markets, triggered by concerns over a strengthening dollar and falling oil prices, had a negative impact.
Among other Asian markets, Hong Kong's Hang Seng was down by 1.09 per cent, while Japan's Nikkei fell 0.09 per cent in early trade today.
The US Dow Jones Industrial Average ended 1.13 per cent lower in yesterday's trade.
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