With a score of 101 against New York's 100, Shanghai rose nine places from last year in cost-of-living ladder.
The survey by 'Economist' Intelligence Unit measured the cost of living in 131 cities worldwide, using New York as the base.
Singapore has been ranked at No 1 position, overtaking Tokyo, driven by a strong currency and high transport costs.
All Chinese cities are moving up in the cost-of-living index, with Shanghai seeing the fastest rise, state-run China Daily reported.
Hong Kong took fifth place in Asia and 13th in the world.
Shenzhen is the second-most-expensive Chinese mainland city, ranked at 39.
"Chinese cities have continued to move up in cost-of-living terms as consumer demand has fed into inflation," said Daili Jon Copestake, editor of the report.
"A mooted slowdown in Chinese growth still represents growth compared with more stagnant mature economies. Wage inflation has driven up prices, but internationally, the impact of a stronger renminbi has also been felt," he said.
Asia still remains host to the world's cheapest cities, most of which are on the Indian subcontinent, with Mumbai being the cheapest.
It aims to provide a guideline to help human resources managers calculate cost-of-living allowances and build compensation packages for expatriates and business travellers.
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