Archbishop William Goh said in a statement posted on the diocese website on Saturday that he had met various government officials to express the church's concerns about the February 28 concert, part of her global Rebel Heart Tour.
The concert, at the 55,000-seat National Stadium, will be Madonna's first-ever in largely conservative Singapore.
She was barred from performing in Singapore in 1993 after police said her performances bordered on the obscene and were "objectionable to many on moral and religious grounds".
Authorities have assured the archbishop that restrictions have been placed to ensure that content deemed offensive to religious beliefs would not be allowed on stage, the church statement said.
The Media Development Authority has restricted the concert to those aged 18 and above because of sexual references.
Local media reports said Madonna would not be performing a controversial tour segment called Holy Water, which includes dancers dressed as scantily-clad nuns performing on cross-shaped stripper poles.
"There is no neutrality in faith; one is either for or against. Being present (at these events) in is itself a counter-witness," the archbishop said.
He warned his flock against supporting "the 'pseudo arts' that promote sensuality, rebellion, disrespect, pornography (and) contamination of the mind of the young".
Some Catholics said they supported the archbishop's decision and would not attend the concert.
Student Kevin Koh, 24, said he would not go but would not pass judgement on fellow Catholics who attend.
Some online comments were critical, with one commentator saying the faithful should be allowed to make their own decisions.
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