The Canadian star's representative confirmed the baby news, reported Digital Spy.
"Mavi Alexandra Jean Amell was born on Tuesday afternoon (October 15). Everyone is happy and healthy," the representative said.
The 32-year-old father posted a photo on his Facebook page of him holding his new born baby daughter.
A caption with the photo read, "She came just in time for Amell Wednesdays."
Amell and his model-actress wife, Jean, tied the knot on vacation in the Caribbean in December 2012, but they decided to re-affirm their commitment to each other in May this year at a ceremony held in New Orleans, Louisiana.