The quake hit at 5:08 am (0308 GMT) with its epicentre just 12 kilometres (7 miles) from the town of Lixourion, 300 kilometres west of Athens, the USGS said.
There were no immediate reports of any damage or casualties.
A 5.8-magnitude earthquake struck the same region late last month.
That quake was felt on several Ionian islands and as far away as the capital Athens.
Greece is one of Europe's most earthquake-prone countries.
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The Mediterranean region as a whole is seismically active due to the convergence of the African and Eurasian tectonic plates.
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