The 32 members of various opposition groups tolerated by the authorities in Damascus and six members of the official Syrian delegation led by the ambassador to the United Nations Bashar Jaafari began their meeting at 0700 GMT (1230 IST), one of the opposition participants told AFP.
The Kremlin-sponsored talks were not expected to yield a major breakthrough as the main opposition group, the exiled National Coalition, has refused to attend.
The leading internationally-recognised opponents of Assad have stayed away, arguing that Russia - one of Assad's few remaining allies - cannot be an honest broker.
The source said that the opposition figures at the meeting would not immediately insist on establishing a transition government to ease Assad from power.
After the meeting this morning, Russia's Foreign Minister Sergei Lavrov was set to meet the Syrian delegation.
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