The tomb was found in Abu-Sir, south-west of Cairo, and is thought to belong to the wife or mother of Pharaoh Neferefre, Egyptian officials said.
Egyptian Antiquities Minister Mamdouh el-Damaty said that her name, Khentakawess, had been found inscribed on a wall in the necropolis.
Damaty added that this would make her Khentakawess III, as two previous queens with the same name have already been identified.
The tomb was discovered in Pharaoh Neferefre's funeral complex.
The Czech archaeologists also found about 30 utensils made of limestone and copper.
The minister said the discovery would "help us shed light on certain unknown aspects of the Fifth Dynasty, which along with the Fourth Dynasty, witnessed the construction of the first pyramids."
Abu-Sir was used as an Old Kingdom cemetery for the ancient Egyptian capital of Memphis.
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