Reza Zarrab is giving evidence in a New York trial over alleged subversion of US economic sanctions against Iran that has implicated former Turkish ministers and even President Recep Tayyip Erdogan.
Erdogan has rubbished the accusations and on Tuesday said that the case was a "plot" aimed at hurting Turkey.
Zarrab, 34, was arrested by US authorities in March 2016 on suspicion of taking part in the alleged sanctions-busting scam but is now cooperating with the authorities in an apparent plea bargain.
A total of 17 people have now been detained as part of this investigation, with three picked up initially and 14 more in a later sweep, the Anadolu news agency reported.
The three detained initially are accused of sending documents to the US regarding the case and links to the group of Fethullah Gulen who Ankara accuses of masterminding the July 15, 2016 failed coup. Gulen denies the charges.
"The case is not a plot against America, it's a plot against Turkey," Erdogan told supporters in a speech on Tuesday.
"The case has nothing to do with law, justice or trade, it's an acrobatic spectacle," he added.
Since Zarrab became a witness, deputy chief executive of Turkish lender Halkbank Mehmet Hakan Atilla is now the lone defendant in the dock accused of violating sanctions, bribery and money laundering.
On Thursday he told the court he was informed that in 2012, then prime minister Erdogan and then treasury minister Ali Babacan had given "instructions" for two other Turkish public banks to take part in the scheme.
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