"The numbers of people fleeing their homes in western Mosul are overwhelming," Lise Grande said in a statement.
"We are talking about very large numbers of families who are leaving everything behind. They are fleeing under very difficult circumstances. Many are food insecure and haven't had access to safe drinking water and medicines for weeks or months," she said.
Iraqi forces launched a massive offensive seven months ago to retake the country's second city from the Islamic State group, which seized it in June 2014.
The government, the UN and its partners have been setting up camps around Mosul to assist increasingly desperate civilians, including some who faced starvation and were used as human shields by jihadists.
But Grande said the aid community was struggling to cope with the latest influx.
"The numbers of people who are moving are now so large, it's becoming more and more difficult to ensure civilians receive the assistance and protection they need," she said.
Grande urged donors to ramp up support for the Mosul aid effort, which has been massively underfunded so far.
"Hundreds of thousands of lives are at stake," she said.
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