The attack in the region's biggest city targeted a camp housing former rebels and pro-government militia who were signatories to a 2015 peace accord struck with the government.
"The United States strongly condemns the cowardly attack on a military camp in Gao that houses government forces and members of the primary armed groups that signed the Malian peace accord," State Department Spokesman Peter Kirby said.
"We are aware of reports that the attack was engineered by a suicide bomber using a truck filled with explosives," his statement said.
The United Nations has deployed 13,000 peacekeeping troops to Mali, but they have repeatedly been targeted in attacks.
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