The lion, which was a popular attraction among many international visitors to the Hwange National Park, was lured outside the park's boundaries by bait and shot earlier this month.
"The offender is a gentleman who is an American called Walter James Palmer," Emmanuel Fundira, president of the Safari Operators Association of Zimbabwe, said at a news conference in the capital Harare.
"A lot of people travel long distances coming to Zimbabwe to enjoy our wildlife and obviously the absence of Cecil is a disaster."
The Zimbabwe Conservation Task Force charity said Palmer and professional Zimbabwean hunter Theo Bronkhorst had gone out at night with a spotlight and tied a dead animal to their vehicle to lure Cecil into range.
"Palmer shot Cecil with a bow and arrow but this shot didn't kill him. They tracked him down and found him 40 hours later when they shot him with a gun," the charity alleged.
It added that the hunters unsuccessfully tried to hide the dead lion's tracking collar, which was part of a University of Cambridge research programme.
Cecil, aged about 13, was said by safari operators to be an "iconic" animal who was recognised by many visitors to Hwange due to his distinctive black mane.
Hwange attracted 50,000 visitors last year, about half of them from abroad.
Bronkhorst and local landowner Honest Ndlovu will appear in court in Victoria Falls on Wednesday to face poaching charges.
"Both the professional hunter and land owner had no permit or quota to justify the offtake of the lion and therefore are liable for the illegal hunt," the Zimbabwean Parks Authority said in a statement Tuesday.
The statement made no mention of Palmer, but added that Bronkhorst's son Zane was also wanted for questioning.
Palmer, whose whereabouts was not known, is an experienced trophy hunter from Minnesota.
He is well-known in US hunting circles as an expert shot with his bow and arrow, and has travelled across the world in pursue of leopards, buffalo rhino, elk and other large mammals.
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