"The pledge to move the embassy is not just an assault against Palestinians but against Arabs and Muslims, who will not remain silent," Muhammad Hussein said in a sermon at Al-Aqsa mosque in Jerusalem's Old City.
On Tuesday, Palestinian leaders called for Friday prayers at mosques across the Middle East this week to protest Trump's campaign pledge.
There have been warnings that the move would constitute recognising Jerusalem as Israel's capital and could inflame tensions in the Middle East and possibly sink what remains of peace efforts.
The Palestinians regard east Jerusalem as the capital of their future state, while Israel proclaims the entire city as its capital.
The city's status is one of the thorniest issues of the decades-long Israeli-Palestinian conflict.
Israel occupied the West Bank and east Jerusalem in 1967. It later annexed east Jerusalem in a move never recognised by the international community.
Mohammad Shtayyeh, a senior Palestinian official and Fatah central committee member, said on Tuesday that the Palestinian leadership had been informed by diplomatic contacts that Trump could call for the move in his inauguration speech on January 20.
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