The broader economic measures will hurt Moscow's ability to secure dollar financing for its banks, in the toughest attack yet on its economy since it became embroiled in the conflict in Ukraine.
"The major sanctions we're announcing today will continue to ratchet up the pressure on Russia, including the cronies and companies supporting Russia's illegal activities in the Ukraine," he said.
Obama denied the West was being drawn into a new Cold War with its former-Soviet foe, but warned that the United States and Europe are running out of patience Vladimir Putin's government.
"We're blocking the exports of specific goods and technologies to the Russian energy sector," he said.
"We're expanding our sanctions to more Russian banks and defence companies. We're formally suspending credits and financing for economic development projects in Russia."
Earlier, the US Treasury had singled out VTB Bank, its subsidiary Bank of Moscow and Russian Agricultural Bank as three institutions joining the existing sanctions regime against Russia.
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