The world trade body's fourth and final verdict against the EU in the dispute opens the way for China to request compensation.
China had first brought the case to the WTO in July 2009 after the EU imposed hefty tariffs on imports of some Chinese steel and iron fasteners.
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The Chinese ministry of commerce said in a statement Monday that the measures had since then had "negative effect on exports from China (of) around $1.0 billion" (918 million euros) and had resulted in some 100,000 people losing their jobs with thousands of fastener producers across the country.
"It has resulted in huge economic losses to the Chinese industry," the statement said.
The WTO has repeatedly found that the EU measures violate global trade rules.
In 2010, a WTO panel ruled that Brussels acted inconsistently in its anti-dumping calculations, and the decision was upheld on appeal in 2011.
But the case did not end there. China did not agree with the EU's claim in October 2012 that it had adjusted its measures to comply with the WTO ruling.
That panel ruled last August in China's favour, finding that the EU had failed to make amends, and on Monday a WTO appeals body rejected an EU appeal of that ruling.
The WTO among other things faulted Brussels for imposing anti-dumping duties on all Chinese firms, instead of imposing differentiated duties depending on each company's practice.
The Chinese commerce ministry statement hailed the ruling, insisting it showed that "discriminative practice and anti-dumping measures taken by certain trade partners against the Chinese products and exporters lack multilateral legal basis under the WTO trade laws".
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