"It will never happen," Mnangagwa said in a speech to his ZANU-PF party supporters in central Zimbabwe, broadcast on television.
His statement comes two months after white farmer Robert Smart got his land back after being evicted in June by ex- president Robert Mugabe's government.
Zimbabwe embarked on a violent land reform programme in 2000, taking over white-owned farms to resettle landless blacks.
Thousands of white farmers were forced off their land by mobs or evicted, with Mugabe saying the reforms would help black people marginalised under British colonial rule.
The government has indicated it will issue 99-year bankable leases to beneficiaries of land reform but Mnangagwa today said land owners must be more productive.
"Our land must be productive. We must mechanise and modernise our agriculture," he said, adding that the land reforms were "irreversible".
Mnangagwa, who came to power after a military intervention ended Mugabe's decades-old rule last year, said new elections would be held in July.
"We want a peaceful election. We want a united people. There is no reason for ZANU-PF to be violent. There is no reason for any political party to be violent."
"Our economy is struggling, unemployment is high, our youth lack opportunities, too many people are unable to afford essential goods for their families and our infrastructure is stuck in the past," he said.
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