Scientists discover fine-tuning gene expression during stress recovery

While getting closer to uncovering a 30-year-old nuclear mystery, scientists have discovered non-coding RNA (Ribonucleic acid), which has a novel role to fine-tune gene expressions during stress recovery.

Hokkaido University researchers are beginning to uncover the functions of mysterious organelles in the nucleus and their relation to stress.

The organelles, called nuclear stress bodies, form when cells are exposed to heat or chemical stress. When conditions return to normal, the organelles promote retention of RNA segments, called introns, the researchers report in The EMBO journal.

This is important because intron retention regulates gene expression for a variety of biological functions including stress response, cell division, learning and memory, preventing the accumulation of damaged DNA, and even tumour growth.

Among their many mysteries, nuclear stress bodies were found to assemble on a type of long non-coding RNA in response to heat and chemical stress. Molecular biologist Tetsuro Hirose of Hokkaido University's Institute for Genetic Medicine specialises in non-coding RNAs, which are molecules copied from DNA, but not translated into proteins. Hirose and his colleagues investigated the functions of nuclear stress bodies by turning off the long non-coding RNA and thus removing them from human cells.

Removing the nuclear stress bodies resulted in a vast suppression of intron retention during stress recovery. Further investigation enabled the team, which included researchers from Hokkaido University, the National Institute for Advanced Industrial Science and Technology, and the University of Tokyo in Japan, to understand how nuclear stress bodies, when they are present, help cells recover from stress.

"Nuclear stress bodies probably function to fine-tune gene expressions by rapidly restoring the proper levels of intron-retaining messenger RNAs as the cell recovers from stress," Tetsuro Hirose says. Further studies are needed to reveal the specific effects of intron retention after heat stress and to understand the detailed mechanism of the process.