"The mission mounted two patrols into Wau today and said it had observed the bodies of 16 civilians in a hospital. There were ten people who had been injured," the statement said.
UNMISS said SPLA troops, tanks and equipment moved towards the south-western part of Wau late last week.
After an ambush south of the city left a number of government SPLA soldiers dead on Sunday, fighting spread to Wau.
Wau is located in a region that has repeatedly changed hands between government troops and rebels loyal to former vice president Riek Machar since the country descended into civil war in 2013.
The town itself is held by forces loyal to President Salva Kiir.
Civil war broke out in South Sudan in 2013 after a power struggle between foes Machar and Kiir.
At least 1.7 million people have fled the country because of the war, and 1.9 million are internally displaced.
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