Al-Qaeda chief Ayman al-Zawahiri had already ordered the group in May 2013 to disband and return to Iraq, and announced that another jihadist group, the Al-Nusra Front, was Al-Qaeda's official branch in war-torn Syria.
The general command of Al-Qaeda rammed home the point in its late yesterday statement.
"Al-Qaeda announces it is not linked to the Islamic State of Iraq and the Levant, as it was not informed of its creation... (and) did not accept it," the statement said.
Jihadists were initially welcomed by some rebels in Syria's conflict, but allegations of brutal abuses against civilians as well as rival opposition fighters has sparked a backlash.
Rebels have accused ISIL of seeking to consolidate power rather than fighting the regime, and even suggested the group was serving the regime's interests.
The tensions erupted in early January into armed clashes between Islamist and moderate rebels and ISIL.
In some cases, Al-Nusra Front participated in clashes against ISIL, though it has largely remained out of the fighting and has called for reconciliation.
