Archaeologists from Florida Atlantic University (FAU) made this discovery just 10 feet below the ground's surface at the Old Vero Man Site in Florida.
The bone was found below a layer that contained material from the Pleistocene period when the last ice age was thought to have occurred, researchers said.
The archaeologists identified the bison using an upper molar, which is thought to be representative of a Bison antiquus, a direct ancestor of the American bison that roamed North America until it became extinct.
"This finding is especially significant because of the meticulous documentation that has been involved," said principal investigator James M Adovasio, from FAU's Harbor Branch Oceanographic Institute.
"Along with the fact that bones like this have never been found on land as part of a calculated archaeological effort. Others like this have all been found underwater, in sinkholes or streams," said Adovasio.
Bison antiquus, sometimes referred to as the "ancient bison," was the most common large herbivore of the North American continent for more than 10,000 years, and is a direct ancestor of the living American bison.
"We couldn't have asked for a better representative species from that era. We now know that people were here in Vero Beach at that time," said lead archaeologist Andrew Hemmings.
Scientists also found other bones at the site from small mammals, along with slivers of bones from large mammals that could have come from mammoth, mastodon, sloth or bison.
Pieces of charcoal and the head of a fly were discovered earlier in this year's excavation, which began in late February.
The Old Man Vero Site was originally discovered in 1915 after construction efforts on a drainage canal exposed the well-preserved remains of late Pleistocene flora and fauna in association with human remains and artifacts.
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