The UN's MINUSMA force head Mongi Hamdi condemned the pro-government Imghad and Allies Tuareg Self-Defence Group (GATIA) which seized key positions in the desert town of Menaka from rebels yesterday.
"I urge the parties to immediately cease hostilities and return to their positions," Hamdi said in a statement, accusing GATIA of "serious violations" of a ceasefire agreement.
"This resurgence of tensions threatens the efforts of all to restore lasting peace in Mali."
The Malian government and a coalition of armed groups from the north known as the Platform had already signed the document, brokered by Algeria under UN auspices over the past eight months.
But the CMA had been holding out, demanding an amendment guaranteeing political recognition for "Azawad", the name used by the Tuareg for the northern part of Mali.
"Months of intense negotiations involving all parties to put an end to the Malian crisis could be threatened," Hamdi said.
The rebels blamed "elements of the Malian army and its militias" for yesterday's attack but the government said in a statement it had learnt of the incident "with great surprise and a strong concern".
It condemned "the resumption of hostilities and the resumption of violence at a time when, with the international community and stakeholders, all efforts are being made to reach a peace agreement".
GATIA told AFP it had only ousted the CMA after the rebels engaged the pro-government group 40 kilometres (25 miles) southwest of Menaka.
Fighting broke out between the army and rebels who had "fired gunshots, without serious consequences", the source said.
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