Police said Hossain, who has played 38 Tests for Bangladesh, and his wife Nritto Shahadat were formally charged on Tuesday with assault and torture.
"The allegations against Shahadat and his wife were proved in our primary investigations," police Inspector Shafiqur Rahman told AFP.
The 29-year-old cricketer, who denies any wrongdoing, was suspended from all forms of the game on September 13 over the allegations.
He and his wife went into hiding after police raided their house in September following a complaint from the young girl.
Hossain, who has also played 51 one-day internationals for Bangladesh, handed himself in to police in October after his wife was arrested.
On Wednesday he told AFP the allegations were "false" and had ruined his career.
"I am innocent. Everyone who knows me will testify to that," he said, in his first interview since his arrest in October.
Hossain said the allegations were part of a conspiracy to destroy his career.
Hossain and his wife face up to 14 years in jail if they are convicted.
