The 25-year old Bhambri overcame a first set lapse to tame Canadian Peter Polansky 1-6 6-3 6-3 in the third and final qualifying round in one hour and 55 minutes.
He will take on Cyprus' seasoned star Marcos Baghdatis, who was a finalist here in 2006.
It will be third Australian Open men's singles main draw for Bhambri who had lost in the first round in 2015 and 2016 to Andy Murray and Tomas Berdych respectively.
At Grand Slams, it is Ramkumar's best show, reaching the final qualifying round.
Bhambri, who was junior world number one, had won Australian Open boys singles tile here in 2009 and has played only at this Grand Slam in the main draw since turning Pro.
Asked what makes him perform at Melbourne Park, Bhambri told PTI," I don't know what makes me play better here. Maybe the conditions suit me or I am just familiar with it because I have played here before."
"I am hoping that I can win a few rounds in the main draw," said Bhambri, who won his sixth ATP Challenger title in Pune in November.
Asked about the first set against the Canadian world number 140, Bhambri said,"It was a nervous start but once I got my rhythm, I started playing more freely.
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